| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Townsend, Rachel A (14)
|
MRBGNW |
25.23 |
26.35 |
1.12 |
| 2 |
Redpath, Frances M (13)
|
MRBGNW |
26.02 |
28.48 |
2.46 |
| 3 |
Nunez, Gabriella A (13)
|
MRBGNW |
28.68 |
28.92 |
0.24 |
| 4 |
Washington, Michelle G (14)
|
MRBGNW |
28.58 |
29.33 |
0.75 |
| 5 |
Sanchez, Samantha M (13)
|
MRBGNW |
29.26 |
29.38 |
0.12 |
| 6 |
DeGeorges, Elizabeth S (13)
|
MRBGNW |
29.71 |
29.88 |
0.17 |
| 7 |
Hulme, Ella R (13)
|
MRBGNW |
30.17 |
29.93 |
-0.24 |
| 8 |
Behbehani, Iman (13)
|
MRBGNW |
30.25 |
30.49 |
0.24 |
| 9 |
Ford, Sydney E (13)
|
MRBGNW |
29.37 |
30.64 |
1.27 |
|
McHugh, Amanda K (14)
|
MRBGNW |
26.91 |
DQ |
- |