| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Guo, Eva J (14)
|
MRLIE |
27.48 |
26.71 |
-0.77 |
| 2 |
Whang, Jessica (14)
|
MRLIE |
27.19 |
26.95 |
-0.24 |
| 3 |
Anderson, Kate M (14)
|
MRLIE |
27.40 |
27.43 |
0.03 |
| 4 |
Huang, Amanda (14)
|
MRLIE |
28.01 |
27.72 |
-0.29 |
| 5 |
Catton, Chloe G (13)
|
MRLIE |
28.02 |
27.90 |
-0.12 |
| 6 |
Jhong, Sara (13)
|
MRLIE |
27.48 |
28.13 |
0.65 |
| 7 |
Perez, Meadow A (14)
|
MRLIE |
27.95 |
28.30 |
0.35 |
| 8 |
Goodstone, Zoe K (14)
|
MRLIE |
28.90 |
29.10 |
0.20 |
| 9 |
Clarkson, Laura A (13)
|
MRLIE |
28.24 |
29.27 |
1.03 |
| 10 |
McDonald, Kelly M (14)
|
MRLIE |
29.74 |
30.05 |
0.31 |
| 11 |
Anderson, Emma R (13)
|
MRLIE |
29.33 |
30.35 |
1.02 |
| 12 |
Calabrese, Victoria (13)
|
MRLIE |
30.73 |
30.50 |
-0.23 |
| 13 |
Campbell, Sarah B (14)
|
MRLIE |
30.91 |
30.79 |
-0.12 |
| 14 |
Prashad, Olivia T (14)
|
MRLIE |
|
31.12 |
- |
| 15 |
Panchal, Jadyn (13)
|
MRLIE |
36.09 |
33.70 |
-2.39 |
| 16 |
Fishler, Arielle E (14)
|
MRLIE |
35.21 |
35.68 |
0.47 |
| 17 |
Walker, Kerri Ann A (13)
|
MRLIE |
|
37.82 |
- |
| 18 |
Akselrod, Abigail (13)
|
MRLIE |
40.88 |
38.11 |
-2.77 |
| 19 |
Ertz, Emily M (13)
|
MRLIE |
|
44.20 |
- |
|
Nascimento, Giovanna (14)
|
MRLIE |
|
NS |
- |
|
Welcome, Shannon R (14)
|
MRLIE |
|
NS |
- |