| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
O'Mara, Erin (8)
|
MRSPAR |
53.35 |
54.01 |
0.66 |
| 2 |
Kung, Alexis (7)
|
MRSPAR |
54.13 |
54.85 |
0.72 |
| 3 |
Finnerty, Katherine J (8)
|
MRAG |
53.40 |
55.47 |
2.07 |
| 4 |
Alexander, Leaila J (7)
|
MRSPAR |
57.98 |
56.40 |
-1.58 |
| 5 |
Regenauer, Magdalena G (8)
|
MRWSSC |
1:03.67 |
57.66 |
-6.01 |
| 6 |
Murray, Megan (8)
|
MRMVA |
1:01.81 |
1:02.46 |
0.65 |
| 7 |
Jankowski, Kaitlyn M (8)
|
MRMVA |
|
1:02.51 |
- |
| 8 |
Vergara, Sofia (8)
|
MRSPAR |
1:02.84 |
1:04.07 |
1.23 |
| 9 |
Kline, Madeline A (8)
|
MRWSSC |
1:10.84 |
1:07.85 |
-2.99 |
| 10 |
Reyes, Sabrina A (8)
|
MRSPAR |
1:15.23 |
1:26.33 |
11.10 |
| 11 |
Brown, Isabella H (7)
|
MRWSSC |
1:09.84 |
1:30.72 |
20.88 |
|
O'Connor, Ryan E (8)
|
MRAG |
55.03 |
DQ |
- |
|
Font, Adrianna (7)
|
MRMVA |
|
DQ |
- |
|
Cachay, Shayla N (6)
|
MRSPAR |
1:15.23 |
DQ |
- |