| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Stalnaker, Taylor M (17)
|
MRAG |
25.13 |
26.42 |
1.29 |
| 2 |
Hutzelmann, Denali M (15)
|
MRAG |
26.14 |
26.66 |
0.52 |
| 3 |
Crumm, Jessica A (18)
|
MRAG |
25.58 |
26.84 |
1.26 |
| 4 |
Flynn, Kate E (16)
|
MRSPAR |
26.92 |
26.94 |
0.02 |
| 5 |
Miller, Molly A (15)
|
MRWSSC |
27.53 |
27.62 |
0.09 |
| 6 |
Johnson, Sydney M (16)
|
MRVAC |
29.50 |
28.80 |
-0.70 |
| 7 |
Johnson, Camryn A (16)
|
MRVAC |
29.29 |
29.37 |
0.08 |
| 8 |
Armstrong, Payton (15)
|
MRUNAG |
29.41 |
29.54 |
0.13 |
| 9 |
Sanchez, Justine A (15)
|
MRVAC |
29.83 |
29.84 |
0.01 |
| 10 |
Feely, Kayleigh E (16)
|
MRAG |
31.98 |
32.45 |
0.47 |
| 11 |
Lewis, Marissa B (16)
|
MRSPAR |
33.98 |
35.09 |
1.11 |
|
Oyen-Ustad, Lillian E (17)
|
MRNDAC |
25.56 |
NS |
- |