| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Valerio, Sara A (8)
|
MRYMID |
39.97 |
37.94 |
-2.03 |
| 2 |
Brennan, Jane E (8)
|
MRRA |
44.20 |
40.54 |
-3.66 |
| 3 |
Wong, Claire (8)
|
NJMALT |
51.61 |
45.60 |
-6.01 |
| 4 |
Fedor, Kate O (8)
|
CTZEUS |
46.36 |
47.16 |
0.80 |
| 5 |
Penn, Haley J (8)
|
MRSMC |
47.93 |
49.00 |
1.07 |
| 6 |
LePage, Charlotte G (8)
|
MRRA |
48.58 |
52.83 |
4.25 |
| 7 |
Amerson, Sydney D (8)
|
MRSMC |
59.99 |
54.53 |
-5.46 |
| 8 |
Jadot, Manon V (8)
|
MREAST |
54.60 |
55.24 |
0.64 |
| 9 |
Carr, Kate A (8)
|
MRRA |
53.05 |
57.63 |
4.58 |
| 10 |
Cann-Polentz, Saihah D (8)
|
MRDA |
|
58.56 |
- |
| 11 |
Fanfarillo, Leila A (8)
|
MREAST |
53.44 |
1:00.12 |
6.68 |
| 12 |
Anselmi, Margeaux L (8)
|
MRCOND |
1:10.00 |
1:00.50 |
-9.50 |
| 13 |
Stillman, Alexandra B (8)
|
MRYFD |
|
1:04.14 |
- |
|
Carousso, Natalie M (8)
|
MRRA |
56.85 |
DQ |
- |
|
Velazquez, Kimberly K (8)
|
CTZEUS |
52.89 |
DQ |
- |