| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Corbitt, Chloe A (14)
|
SCH2A |
|
57.10 |
- |
| 2 |
Pearce, Lucy S (16)
|
NCSGSA |
|
57.22 |
- |
| 3 |
Newnam, Anna S (15)
|
NCWNCY |
|
57.44 |
- |
| 4 |
Arens, Abigail L (13)
|
NCMOR |
|
57.48 |
- |
| 5 |
Clayton-Luce, Sophia H (15)
|
NCWAVE |
|
57.90 |
- |
| 6 |
Clabeaux, Jacqueline R (16)
|
PVYORK |
|
58.09 |
- |
| 7 |
Cottle, Elizabeth A (14)
|
FLSTAR |
|
58.42 |
- |
| 8 |
Diaz, Alicia (17)
|
MAKAY |
|
58.51 |
- |
| 9 |
Ritz, Erin (14)
|
NIVICT |
|
58.64 |
- |
| 10 |
Dong, Yunfan (14)
|
ADSCHE |
|
58.76 |
- |
| 11 |
Vitolins, Anna M (17)
|
ADSCHE |
|
59.59 |
- |
| 12 |
Klafehn, Bailey R (16)
|
NIVICT |
|
1:00.64 |
- |
| 13 |
Lynch, Kathleen M (17)
|
LELESD |
|
1:02.32 |
- |