| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gomez Treig, Gabriel A (16)
|
FLBSS |
52.00 |
50.17 |
-1.83 |
| 2 |
Huang, James J (16)
|
CTUN-0 |
50.48 |
50.42 |
-0.06 |
| 3 |
Mayfield, Alexander L (17)
|
GALINS |
51.00 |
50.85 |
-0.15 |
| 4 |
Essary, Tristan B (18)
|
SEPSL |
50.02 |
50.87 |
0.85 |
| 5 |
Dalmolin, Jack F (16)
|
GAGOLD |
1:00.00 |
50.97 |
-9.03 |
| 6 |
Hollinshead, Henry C (17)
|
NCYOTA |
51.99 |
51.11 |
-0.88 |
| 7 |
Tempkin, Maxwell M (18)
|
FLBSS |
52.00 |
51.23 |
-0.77 |
| 8 |
Plant, Nolan J (17)
|
MDJCC |
51.03 |
51.32 |
0.29 |
| 9 |
Froass, Dillon C (16)
|
OHCM |
51.50 |
52.04 |
0.54 |
| 10 |
Stabler, Andrew B (16)
|
SEAUB |
51.00 |
53.04 |
2.04 |
| 11 |
Garbee, Stefan M (16)
|
CTUN-0 |
55.00 |
54.46 |
-0.54 |