| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lee, Sophia (10)
|
NIUN-D |
39.12 |
38.54 |
-0.58 |
| 2 |
Murphy, Abbie (10)
|
NIVICT |
41.58 |
40.32 |
-1.26 |
| 3 |
Reilly, Fiona K (10)
|
NISTAR |
41.36 |
41.20 |
-0.16 |
| 4 |
Marcyan, Chloe M (9)
|
NITTSC |
42.60 |
43.08 |
0.48 |
| 5 |
Keller, Mira (10)
|
NIVELO |
43.68 |
43.35 |
-0.33 |
| 6 |
Er, Melissa (9)
|
NIUN-D |
57.97 |
44.50 |
-13.47 |
| 7 |
Moldenhauer, Paige A (10)
|
NIEAST |
47.68 |
46.10 |
-1.58 |
| 8 |
Barry, Madeline G (10)
|
NIVICT |
55.61 |
46.77 |
-8.84 |
| 9 |
Wamsley, Sarah E (9)
|
NIVELO |
50.25 |
48.12 |
-2.13 |
| 10 |
Mintz, Vanessa M (10)
|
NICLSC |
48.46 |
48.67 |
0.21 |
| 11 |
Philbrick, Mylie E (9)
|
NIVELO |
50.92 |
48.99 |
-1.93 |
| 12 |
Adamczyk, Gabrielle J (10)
|
NICLSC |
|
49.60 |
- |
| 13 |
Onze, Lauren W (9)
|
NIVICT |
49.25 |
50.23 |
0.98 |
| 14 |
Kreinheder, Sophie A (10)
|
NISTAR |
|
51.10 |
- |
| 15 |
Wagner, Morgan E (9)
|
NIVICT |
55.97 |
51.32 |
-4.65 |
| 16 |
Gachette, Marisa B (9)
|
NIVELO |
51.32 |
52.81 |
1.49 |
| 17 |
Sperry, Lola E (9)
|
NITTSC |
58.37 |
54.32 |
-4.05 |
| 18 |
Reukauf, Anna R (9)
|
NISTAR |
52.30 |
54.36 |
2.06 |
| 19 |
Prezyna, Zoey (9)
|
NIVICT |
56.07 |
56.49 |
0.42 |
| 20 |
Onze, Emily S (9)
|
NIVICT |
1:10.02 |
59.24 |
-10.78 |
| 21 |
Tothero, Madeleine S (9)
|
NISTAR |
55.08 |
59.83 |
4.75 |
| 22 |
Schmitt, Karli (10)
|
NITTSC |
|
1:02.37 |
- |
| 23 |
Strittmatter, Amelia C (9)
|
NISTAR |
1:02.29 |
1:04.84 |
2.55 |
| 24 |
Kessel, Grace E (10)
|
NISHAQ |
1:03.32 |
1:05.38 |
2.06 |
| 25 |
DelRegno, Isabella M (10)
|
NIVELO |
|
1:08.14 |
- |
| 26 |
Kauffman, Faith M (9)
|
NISTAR |
1:09.40 |
1:08.33 |
-1.07 |
|
Hesch, Addison L (9)
|
NITTSC |
|
DQ |
- |
|
Weisenburg, Brylee M (9)
|
NISTAR |
58.30 |
DQ |
- |
|
Stevens, Eleanor R (9)
|
NISTAR |
|
DQ |
- |
|
Brassard, Addison G (10)
|
NISTAR |
52.85 |
DQ |
- |