| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Keller, Mira (10)
|
NIVELO |
33.95 |
33.59 |
-0.36 |
| 2 |
Reilly, Fiona K (10)
|
NISTAR |
36.26 |
36.27 |
0.01 |
| 3 |
Adamczyk, Gabrielle J (10)
|
NICLSC |
38.82 |
38.28 |
-0.54 |
| 4 |
Murphy, Abbie (10)
|
NIVICT |
47.02 |
38.52 |
-8.50 |
| 5 |
Mintz, Vanessa M (10)
|
NICLSC |
39.62 |
40.80 |
1.18 |
| 6 |
Er, Melissa (9)
|
NIUN-D |
55.00 |
40.83 |
-14.17 |
| 7 |
Onze, Lauren W (9)
|
NIVICT |
47.31 |
43.92 |
-3.39 |
| 8 |
Prezyna, Zoey (9)
|
NIVICT |
47.11 |
44.03 |
-3.08 |
| 9 |
Barry, Madeline G (10)
|
NIVICT |
48.40 |
45.26 |
-3.14 |
| 10 |
Barillari, Emma (9)
|
NIVELO |
45.82 |
47.00 |
1.18 |
| 11 |
Philbrick, Mylie E (9)
|
NIVELO |
52.88 |
47.04 |
-5.84 |
| 12 |
McKinney, Madison N (10)
|
NICLSC |
50.27 |
47.25 |
-3.02 |
| 13 |
Kreinheder, Sophie A (10)
|
NISTAR |
49.57 |
48.98 |
-0.59 |
| 14 |
Wamsley, Sarah E (9)
|
NIVELO |
51.46 |
49.24 |
-2.22 |
| 15 |
Moldenhauer, Paige A (10)
|
NIEAST |
49.56 |
53.72 |
4.16 |
| 16 |
Reukauf, Anna R (9)
|
NISTAR |
1:00.53 |
1:06.58 |
6.05 |
| 17 |
Kauffman, Faith M (9)
|
NISTAR |
1:14.37 |
1:17.13 |
2.76 |
|
Fisk, Hailey E (9)
|
NISHAQ |
|
DQ |
- |
|
Brassard, Addison G (10)
|
NISTAR |
58.81 |
DQ |
- |