| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Naccarella, Maria E (14)
|
NIWEBS |
2:27.40 |
2:24.90 |
-2.50 |
| 2 |
Taberski, Kathleen M (13)
|
NISDSC |
|
2:29.04 |
- |
| 3 |
Federico, Sydney A (14)
|
NIGRSC |
2:43.29 |
2:40.09 |
-3.20 |
| 4 |
Kocher, Casey A (14)
|
NISDSC |
2:39.78 |
2:40.39 |
0.61 |
| 5 |
Wallace, Emma E (13)
|
NIWEBS |
2:46.39 |
2:45.59 |
-0.80 |
| 6 |
Kennedy, Madaliene F (14)
|
NISDSC |
2:51.71 |
2:47.82 |
-3.89 |
| 7 |
Burns, Hannah B (13)
|
NIGRSC |
|
2:51.65 |
- |
| 8 |
Richards, Amelia L (13)
|
NIGRSC |
|
2:54.12 |
- |
| 9 |
Brooks, Mackenzie N (13)
|
NIWEBS |
3:26.28 |
2:59.85 |
-26.43 |
|
Ingraham, Leah M (13)
|
NIGRSC |
2:29.01 |
NS |
- |