| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bierley, Benjamin R (10)
|
NISDSC |
33.21 |
34.81 |
1.60 |
| 2 |
Eagley, Wyatt T (9)
|
NIPMSC |
38.24 |
36.94 |
-1.30 |
| 3 |
Garnot, Griffin W (10)
|
NIWEBS |
37.14 |
37.83 |
0.69 |
| 4 |
Evans, Ethan D (9)
|
NIGRSC |
37.15 |
39.87 |
2.72 |
| 5 |
Warren, Wesley C (9)
|
NIWEBS |
37.05 |
40.47 |
3.42 |
| 6 |
Quigley, Kian C (10)
|
NIGRSC |
44.44 |
41.52 |
-2.92 |
| 7 |
Vallow, Brayden T (10)
|
NISDSC |
44.71 |
43.48 |
-1.23 |
| 8 |
Twohig, Colin M (9)
|
NIGRSC |
44.87 |
45.39 |
0.52 |
| 9 |
Newsome, George W (9)
|
NIPMSC |
49.67 |
46.54 |
-3.13 |
| 10 |
Richards-Dyson, Jax E (9)
|
NIGCOM |
|
50.60 |
- |
| 11 |
Cottone, Cameron A (9)
|
NIGCOM |
52.46 |
55.09 |
2.63 |
| 12 |
Ellickson, Cameron B (10)
|
NISDSC |
1:06.18 |
57.09 |
-9.09 |
|
Holly, Gaige W (10)
|
NIGCOM |
54.15 |
NS |
- |