| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gillan, Sierra P (12)
|
MAPFY |
28.26 |
30.04 |
1.78 |
| 2 |
Hartmann, Sierra F (11)
|
MAPFY |
35.37 |
33.41 |
-1.96 |
| 3 |
Fleischaker, Leyna S (12)
|
MAPAAC |
35.06 |
34.15 |
-0.91 |
| 4 |
Newton, Emily M (12)
|
MAUN |
|
34.69 |
- |
| 5 |
Walls, Lauren R (11)
|
MAPAAC |
34.17 |
35.55 |
1.38 |
| 6 |
Sheridan, Sophie E (12)
|
MAPAAC |
34.16 |
35.79 |
1.63 |
| 7 |
Szerencsits, Olivia S (12)
|
MAPAAC |
36.74 |
35.87 |
-0.87 |
| 8 |
Gillan, Madison F (11)
|
MAPFY |
35.46 |
36.26 |
0.80 |
| 9 |
Sheridan, Isabel L (12)
|
MAPAAC |
35.80 |
36.93 |
1.13 |
| 10 |
Dolan, Rachel M (12)
|
MAPAAC |
40.22 |
39.84 |
-0.38 |
| 11 |
Mehta, Tanya (12)
|
MAPAAC |
38.78 |
39.97 |
1.19 |
| 12 |
Bower, Skyler (12)
|
MAUN |
38.64 |
40.40 |
1.76 |
|
Lubbe, Chelsea (12)
|
MAPFY |
44.70 |
NS |
- |