| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gendzel, Maya C (11)
|
MASSC |
30.36 |
29.49 |
-0.87 |
| 2 |
Barrett, Jamie L (12)
|
MASSC |
30.36 |
30.74 |
0.38 |
| 3 |
Ott, Madeleine F (12)
|
MASSC |
32.20 |
32.20 |
0.00 |
| 4 |
Eruslanova, Ekaterina (11)
|
MASSC |
33.47 |
32.25 |
-1.22 |
| 5 |
Whitaker, Kallie V (12)
|
MASSC |
32.92 |
32.43 |
-0.49 |
| 6 |
Feuzeu Mekoue, Laurielle Kel (11)
|
MASSC |
32.17 |
32.51 |
0.34 |
| 7 |
Leather, Alice G (12)
|
MABSC |
34.27 |
32.62 |
-1.65 |
| 8 |
Bailey, Elishka S (12)
|
MABSC |
34.36 |
33.04 |
-1.32 |
| 9 |
Shifflett, Ella M (11)
|
MASSC |
35.82 |
34.90 |
-0.92 |
| 10 |
Levensten, Kate E (11)
|
MASSC |
35.50 |
35.68 |
0.18 |
| 11 |
Isaacs, Eve A (11)
|
MABSC |
37.75 |
37.20 |
-0.55 |
| 12 |
Levensten, Sydney J (11)
|
MASSC |
35.86 |
38.53 |
2.67 |
| 13 |
Scott, Emily B (12)
|
MAUNAT |
45.49 |
40.54 |
-4.95 |
| 14 |
Wert, Kathryn D (11)
|
MABSC |
|
41.05 |
- |
| 15 |
Burnetta, Sabrina C (11)
|
MABSC |
40.35 |
41.47 |
1.12 |
| 16 |
Markakis, Georgia (11)
|
MATRI |
39.90 |
41.78 |
1.88 |
| 17 |
McDonnell, Lilly A (12)
|
MATRI |
42.46 |
44.68 |
2.22 |
| 18 |
Kelly, Ava L (11)
|
MABSC |
48.18 |
44.87 |
-3.31 |
| 19 |
Lorei, Violet C (11)
|
MABSC |
|
46.04 |
- |
| 20 |
Chapin, Brooke O (11)
|
MATRI |
45.73 |
46.28 |
0.55 |
| 21 |
Murnaghan, Ella A (11)
|
MASSC |
51.92 |
46.72 |
-5.20 |
|
Furst, Josie M (11)
|
MATRI |
|
DQ |
- |