| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Brennan, Reilly E (12)
|
MASSC |
28.86 |
29.44 |
0.58 |
| 2 |
Feuzeu Mekoue, Laurielle Kel (11)
|
MASSC |
30.81 |
29.54 |
-1.27 |
| 3 |
Kolessar, Ashley O (11)
|
MASSC |
31.23 |
30.25 |
-0.98 |
| 4 |
Ott, Madeleine F (12)
|
MASSC |
31.14 |
30.75 |
-0.39 |
| 5 |
Eruslanova, Ekaterina (11)
|
MASSC |
30.73 |
30.97 |
0.24 |
| 6 |
Barrett, Jamie L (12)
|
MASSC |
31.15 |
31.38 |
0.23 |
| 7 |
Waite, Katharine M (12)
|
MASSC |
31.58 |
31.41 |
-0.17 |
| 8 |
Bailey, Elishka S (12)
|
MABSC |
34.09 |
31.98 |
-2.11 |
| 9 |
Levensten, Kate E (11)
|
MASSC |
33.41 |
32.83 |
-0.58 |
| 10 |
Leather, Alice G (12)
|
MABSC |
32.21 |
32.85 |
0.64 |
| 11 |
Whitaker, Kallie V (12)
|
MASSC |
32.95 |
33.05 |
0.10 |
| 12 |
Holtsberg, Cathryn F (11)
|
MASSC |
34.41 |
33.60 |
-0.81 |
| 13 |
Agostini, Natalie T (12)
|
MABSC |
35.01 |
33.75 |
-1.26 |
| 14 |
Isaacs, Eve A (11)
|
MABSC |
36.62 |
34.08 |
-2.54 |
| 15 |
Becker, Mandy C (12)
|
MABSC |
34.09 |
34.14 |
0.05 |
| 16 |
Levensten, Sydney J (11)
|
MASSC |
33.76 |
34.50 |
0.74 |
| 17 |
Lissack, Julia C (11)
|
MABSC |
40.68 |
39.56 |
-1.12 |
| 18 |
Burnetta, Sabrina C (11)
|
MABSC |
41.17 |
42.60 |
1.43 |
| 19 |
Kelly, Ava L (11)
|
MABSC |
45.49 |
43.50 |
-1.99 |
| 20 |
Lorei, Violet C (11)
|
MABSC |
|
46.71 |
- |
| 21 |
Murnaghan, Ella A (11)
|
MASSC |
59.99 |
49.86 |
-10.13 |
|
Furst, Josie M (11)
|
MATRI |
|
DQ |
- |