| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Famous, Caroline A (15)
|
MASSC |
51.03 |
52.96 |
1.93 |
| 2 |
Martin, Summer M (17)
|
MASSC |
51.70 |
54.77 |
3.07 |
| 3 |
Paglia, Sydney J (14)
|
MASSC |
52.58 |
55.75 |
3.17 |
| 4 |
Neveling, Abbey E (18)
|
MAJW |
52.08 |
56.61 |
4.53 |
| 5 |
Woods, Nora C (16)
|
MAJW |
54.90 |
58.06 |
3.16 |
| 6 |
Raue, Madeline A (17)
|
MAJW |
55.08 |
59.15 |
4.07 |
| 7 |
Stansfield, Jordan E (17)
|
MASSC |
54.47 |
59.31 |
4.84 |
| 8 |
Jensen, Erin E (14)
|
MAJW |
57.96 |
59.80 |
1.84 |
| 9 |
Apathy, Caroline E (18)
|
MASSC |
54.33 |
1:00.44 |
6.11 |
| 10 |
Diamandi, Michelle I (15)
|
MASSC |
59.60 |
1:02.28 |
2.68 |
| 11 |
Jablonski, Camille E (17)
|
MASSC |
54.54 |
1:03.73 |
9.19 |
| 12 |
Diamandi, Julia A (15)
|
MASSC |
59.44 |
1:04.33 |
4.89 |
|
Dickert, Madison R (15)
|
MASSC |
58.36 |
NS |
- |
|
Homitz, Jessica L (17)
|
MASSC |
55.48 |
NS |
- |