| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Famous, Caroline A (15)
|
MASSC |
23.02 |
24.09 |
1.07 |
| 2 |
Paglia, Sydney J (14)
|
MASSC |
24.00 |
25.30 |
1.30 |
| 3 |
Cooke, Madeleine E (17)
|
MASSC |
23.30 |
25.36 |
2.06 |
| 4 |
Neveling, Abbey E (18)
|
MAJW |
23.77 |
25.38 |
1.61 |
| 5 |
Cavanagh, Lauren E (17)
|
MAJW |
25.82 |
26.30 |
0.48 |
| 6 |
Jablonski, Camille E (17)
|
MASSC |
25.51 |
26.42 |
0.91 |
| 7 |
Stansfield, Jordan E (17)
|
MASSC |
25.01 |
27.09 |
2.08 |
| 8 |
Raue, Madeline A (17)
|
MAJW |
25.70 |
27.20 |
1.50 |
| 9 |
Jensen, Erin E (14)
|
MAJW |
26.36 |
27.29 |
0.93 |
| 10 |
Bergstrom, Sydney L (12)
|
MASSC |
26.17 |
27.42 |
1.25 |
| 11 |
Diamandi, Michelle I (15)
|
MASSC |
26.88 |
28.04 |
1.16 |
| 12 |
Chang, Erin R (16)
|
MASSC |
27.33 |
28.27 |
0.94 |
|
Dickert, Madison R (15)
|
MASSC |
27.31 |
NS |
- |
|
Homitz, Jessica L (17)
|
MASSC |
24.85 |
NS |
- |