| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ketron, Anna E (15)
|
MRBGNW |
25.66 |
27.06 |
1.40 |
| 2 |
Dubin, Karen E (16)
|
MRBGNW |
26.57 |
27.28 |
0.71 |
| 3 |
Zhu, Catherine (15)
|
MRBGNW |
27.88 |
28.10 |
0.22 |
| 4 |
McHugh, Amanda K (16)
|
MRBGNW |
26.44 |
28.23 |
1.79 |
| 5 |
Kleinsmith, Margaret T (15)
|
MRBGNW |
26.37 |
28.98 |
2.61 |
| 6 |
Sanchez, Samantha M (15)
|
MRBGNW |
28.88 |
29.98 |
1.10 |
| 7 |
Garofolo, Juliette M (16)
|
MRBGNW |
28.97 |
30.34 |
1.37 |
| 8 |
Ford, Sydney E (15)
|
MRBGNW |
28.56 |
30.57 |
2.01 |
| 9 |
Behbehani, Iman (15)
|
MRBGNW |
29.50 |
30.98 |
1.48 |
| 10 |
Cahaly, Emma E (15)
|
MRYMID |
32.10 |
31.78 |
-0.32 |
| 11 |
Medev, Gabrielle (15)
|
MRSHY |
34.00 |
34.00 |
0.00 |
|
Belostotsky, Hannah F (11)
|
MRYMID |
38.02 |
NS |
- |
|
Lopez, Maria Camila (12)
|
MRYMID |
36.95 |
NS |
- |