| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Phelan, Denise G (11)
|
MRTS |
37.24 |
36.81 |
-0.43 |
| 2 |
Donlevy, Margaret M (12)
|
MRCIY |
38.18 |
36.83 |
-1.35 |
| 3 |
Londono, Isabela (12)
|
MRIA |
40.22 |
39.07 |
-1.15 |
| 4 |
Horan, Brenna (12)
|
MRIA |
40.83 |
39.77 |
-1.06 |
| 5 |
Kazlauskas, Saige (12)
|
MRHAA |
39.00 |
39.97 |
0.97 |
| 6 |
Johannsen, Erin (11)
|
MRHAA |
39.97 |
40.80 |
0.83 |
| 7 |
Modni, Hanna R (12)
|
MRBBSC |
39.93 |
41.06 |
1.13 |
| 8 |
Dobrzynski, Tercia Z (12)
|
MRIA |
40.14 |
42.08 |
1.94 |
| 9 |
Maio, Kate (12)
|
MRBBSC |
41.66 |
42.58 |
0.92 |
| 10 |
McNamara, Jane (12)
|
MRBBSC |
42.07 |
43.01 |
0.94 |
| 11 |
Wojciechowski, Natalia (11)
|
MRIA |
44.13 |
44.00 |
-0.13 |
| 12 |
Libohova, Anna (12)
|
MRTVSC |
43.77 |
44.72 |
0.95 |
| 13 |
Combs, Elizabeth (12)
|
MRHAA |
45.28 |
45.37 |
0.09 |
| 14 |
Dansker, Renee M (11)
|
MRCIY |
46.16 |
46.32 |
0.16 |
| 15 |
Claps, Abigail R (11)
|
MRHAA |
47.56 |
47.89 |
0.33 |
| 16 |
Kohli, Diya (11)
|
MRTS |
52.97 |
49.51 |
-3.46 |
| 17 |
Kukreja, Samara (12)
|
MRTS |
49.69 |
50.97 |
1.28 |
| 18 |
DeSantis, Lauren N (11)
|
MRIA |
48.96 |
51.24 |
2.28 |
|
Schubel, Sarah K (12)
|
MRHAA |
|
DQ |
- |
|
Park, Emma J (11)
|
MRIA |
49.96 |
DQ |
- |
|
Barsony, Amber (11)
|
MRHAA |
41.64 |
DQ |
- |