| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
O'Shea, Madaghan L (10)
|
MRPBAC |
36.86 |
36.79 |
-0.07 |
| 2 |
Bryant, Jordyn M (10)
|
MRNBS |
39.50 |
37.87 |
-1.63 |
| 3 |
Bilenky, Silvia (10)
|
MRNBS |
40.32 |
40.70 |
0.38 |
| 4 |
Regenauer, Magdalena G (10)
|
MRNBS |
42.95 |
41.26 |
-1.69 |
| 5 |
DeGeorge, Sadie (9)
|
MRPBAC |
45.46 |
41.98 |
-3.48 |
| 6 |
Leonard, Julia E (10)
|
MRNBS |
45.59 |
45.39 |
-0.20 |
| 7 |
Magnetico, Rebecca G (10)
|
MRPBAC |
47.63 |
47.51 |
-0.12 |
| 8 |
Murray, Megan (10)
|
MRMVA |
46.77 |
49.02 |
2.25 |
| 9 |
Mastandrea, Amanda (10)
|
MRMVA |
49.47 |
49.95 |
0.48 |
| 10 |
Alston, Makayla M (10)
|
MRNBS |
|
51.68 |
- |
| 11 |
Losgar, Morghain (9)
|
MRNBS |
54.55 |
53.64 |
-0.91 |
| 12 |
Leonard, Emily V (9)
|
MRNBS |
54.92 |
54.30 |
-0.62 |
| 13 |
Portelli, Samantha F (9)
|
MRPBAC |
1:35.12 |
55.78 |
-39.34 |
| 14 |
Wallace, Olivia (10)
|
MRMVA |
|
58.56 |
- |
| 15 |
Riccardi, Rachael A (9)
|
MRNBS |
|
1:06.22 |
- |
|
Haydt, Isabella H (10)
|
MRUN |
|
DQ |
- |