| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Soule, Mary O (17)
|
NCASC |
25.00 |
25.72 |
0.72 |
| 2 |
Bowers, Anne C (18)
|
NCASC |
25.52 |
27.12 |
1.60 |
| 3 |
Bauer, Katherine M (13)
|
NCASC |
28.10 |
28.21 |
0.11 |
| 4 |
Ungert, Anna-Olivia M (13)
|
NCASC |
28.62 |
28.54 |
-0.08 |
| 5 |
Davis, Tessa M (17)
|
NCSMAC |
28.03 |
28.67 |
0.64 |
| 6 |
Mack, Isabel G (16)
|
NCASC |
28.40 |
29.96 |
1.56 |
| 7 |
Payne, Victoria N (17)
|
NCASC |
28.78 |
30.34 |
1.56 |
| 8 |
Butler, Erin K (16)
|
NCASC |
28.61 |
31.97 |
3.36 |
| 9 |
Wagenseil, Barrett W (14)
|
NCSMAC |
36.65 |
34.36 |
-2.29 |
| 10 |
Gwan-Nulla, Emma G (13)
|
NCSMAC |
36.80 |
36.45 |
-0.35 |