| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rednour, Austin T (21)
|
NCSAC |
52.05 |
53.98 |
1.93 |
| 2 |
Fender, Matthew J (16)
|
NCGG |
59.56 |
57.21 |
-2.35 |
| 3 |
Anderson, William T (18)
|
NCGG |
57.83 |
57.55 |
-0.28 |
| 4 |
McRea, Micah W (15)
|
NCCVAC |
56.90 |
58.84 |
1.94 |
| 5 |
Leatherman, Brady H (15)
|
NCCVAC |
56.93 |
59.03 |
2.10 |
| 6 |
Murphy, Samuel A (17)
|
NCLENR |
56.13 |
59.20 |
3.07 |
| 7 |
Helms, Brice A (16)
|
NCWA |
57.75 |
1:00.45 |
2.70 |
| 8 |
Welch, Riley T (16)
|
NCMSC |
1:03.57 |
1:02.10 |
-1.47 |
| 9 |
Martin, Micah R (15)
|
NCCVAC |
1:06.34 |
1:04.41 |
-1.93 |
| 10 |
Patterson, William E (16)
|
NCSAC |
|
1:05.48 |
- |
| 11 |
Sacchetti, Mario T (15)
|
NCMSC |
1:06.07 |
1:05.83 |
-0.24 |
| 12 |
Guerriero, Nicholas D (16)
|
NCSAC |
1:04.11 |
1:06.47 |
2.36 |
| 13 |
Teague, Jeffery B (15)
|
NCWA |
1:04.13 |
1:07.79 |
3.66 |
| 14 |
Carroll, Andrew C (16)
|
NCBAD |
1:06.91 |
1:08.58 |
1.67 |
| 15 |
Sebastian, William K (17)
|
NCLENR |
1:08.49 |
1:10.31 |
1.82 |
| 16 |
Bauer, Aaron C (17)
|
NCLENR |
1:06.13 |
1:12.17 |
6.04 |
| 17 |
Geist, Keethan A (15)
|
NCNMA |
1:14.17 |
1:13.69 |
-0.48 |
| 18 |
Walker, Jalon M (18)
|
NCLENR |
1:07.71 |
1:16.18 |
8.47 |
| 19 |
Jones, Chase O (17)
|
NCGG |
1:09.25 |
1:17.04 |
7.79 |
| 20 |
Smith, Lucas A (13)
|
NCGG |
1:34.99 |
1:21.07 |
-13.92 |
| 21 |
Cooper, Christian B (17)
|
NCWA |
|
1:22.27 |
- |
|
Meagher, Will L (24)
|
NCWA |
52.66 |
NS |
- |