| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wolk, Audrey J (10)
|
NCNCAC |
41.09 |
39.93 |
-1.16 |
| 2 |
Burton, Lillian H (10)
|
NCNCAC |
43.33 |
41.39 |
-1.94 |
| 3 |
Foreman, Riley M (9)
|
NCMOR |
42.72 |
43.43 |
0.71 |
| 4 |
Herring, Kate E (10)
|
NCMOR |
44.18 |
43.59 |
-0.59 |
| 5 |
Pezzimenti, Alessandra E (10)
|
PVMACH |
47.09 |
44.38 |
-2.71 |
| 6 |
Su, Alicia (9)
|
NCNCAC |
47.47 |
44.50 |
-2.97 |
| 7 |
Lloyd, Kyley B (9)
|
NCNCAC |
45.49 |
44.95 |
-0.54 |
| 8 |
Kearns, Amanda N (10)
|
NCMOR |
47.68 |
46.49 |
-1.19 |
| 9 |
Ray, Molly K (10)
|
NCMOR |
47.70 |
48.08 |
0.38 |
| 10 |
Webb, Margaret C (10)
|
NCMOR |
46.04 |
48.15 |
2.11 |
| 11 |
Takei, Kamiko V (9)
|
NCMOR |
49.89 |
49.99 |
0.10 |
| 12 |
Sciaudone, Gabrielle S (9)
|
NCMOR |
56.60 |
52.88 |
-3.72 |
| 13 |
Bondell, Josephine A (9)
|
NCMOR |
53.58 |
52.98 |
-0.60 |
| 14 |
Saunders, Caitlin (10)
|
PVMACH |
53.16 |
54.99 |
1.83 |
|
Charters, Shannon A (9)
|
PVMACH |
47.30 |
DQ |
- |
|
Miller, Jessica M (8)
|
NCUN |
53.93 |
DQ |
- |
|
Light, Chloe C (8)
|
NCMOR |
46.06 |
NS |
- |