| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bryant, Katherine R (11)
|
NCRSA |
34.40 |
32.01 |
-2.39 |
| 2 |
Rydzewski, Samantha G (10)
|
NCRSA |
35.20 |
35.16 |
-0.04 |
| 3 |
Riley, Erin N (10)
|
NCRSA |
33.91 |
35.67 |
1.76 |
| 4 |
Maleski, Mary C (12)
|
NCRSA |
37.20 |
36.39 |
-0.81 |
| 5 |
Lu, Angelina Z (8)
|
NCRSA |
40.81 |
37.37 |
-3.44 |
| 6 |
Malley, Delaney L (11)
|
NCRSA |
43.03 |
38.71 |
-4.32 |
| 7 |
Holloway, Blythe M (10)
|
NCRSA |
41.32 |
39.11 |
-2.21 |
| 8 |
Waldschmidt, Madison O (10)
|
NCRSA |
38.31 |
39.66 |
1.35 |
| 9 |
Hadsell, Meghan L (8)
|
NCRSA |
|
43.24 |
- |
| 10 |
Shen, Katie L (10)
|
NCRSA |
|
43.54 |
- |
| 11 |
Andrews, Stella E (10)
|
NCRSA |
45.81 |
43.55 |
-2.26 |
| 12 |
Johnson, Addison L (10)
|
NCRSA |
46.39 |
46.21 |
-0.18 |
| 13 |
Chen, Sarah Y (8)
|
NCRSA |
49.71 |
47.01 |
-2.70 |
| 14 |
Hoang, Christiana E (6)
|
NCRSA |
|
55.55 |
- |
|
Humphrey, Persephone R (10)
|
NCRSA |
54.72 |
DQ |
- |