| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Frank, Jack P (10)
|
NCCAT |
2:33.01 |
2:27.04 |
-5.97 |
| 2 |
Diacumakos, Nicholas R (10)
|
NCRSA |
2:28.52 |
2:27.24 |
-1.28 |
| 3 |
Jones, Griffin D (10)
|
NCSFSC |
2:32.20 |
2:31.45 |
-0.75 |
| 4 |
Hughes, Jackson C (10)
|
NCWOW |
2:34.89 |
2:32.24 |
-2.65 |
| 5 |
McNabb, Zachary M (9)
|
VAODAC |
2:38.15 |
2:38.88 |
0.73 |
| 6 |
Agans, Nikhil S (10)
|
NCCAT |
2:34.24 |
2:39.39 |
5.15 |
| 7 |
March, Jackson C (9)
|
NCRSA |
2:38.05 |
2:39.58 |
1.53 |
| 8 |
Holtham, Isaac O (10)
|
NCMSA |
|
2:49.76 |
- |
| 9 |
Kuzma, Charlie R (10)
|
NCSSS |
2:53.58 |
2:50.78 |
-2.80 |
| 10 |
Webb, Davis J (10)
|
NCRSA |
2:55.63 |
2:51.71 |
-3.92 |
| 11 |
Koepp, Tyler S (9)
|
VAODAC |
3:02.96 |
2:57.80 |
-5.16 |
| 12 |
Niebauer, Robert E (8)
|
NCHPSC |
|
2:57.94 |
- |
| 13 |
Conklin, Brandt C (9)
|
NCSFSC |
3:04.86 |
3:02.20 |
-2.66 |
| 14 |
Salgado, Wayne J (10)
|
VAODAC |
3:11.40 |
3:08.00 |
-3.40 |
| 15 |
Lee, Truman R (8)
|
NCSAIL |
|
3:10.90 |
- |
| 16 |
Mejouev, Maxim (10)
|
NCWOW |
3:58.71 |
3:15.90 |
-42.81 |
| 17 |
Bernstein, Carson G (10)
|
VAODAC |
3:23.47 |
3:25.10 |
1.63 |
| 18 |
Christensen, Matias M (9)
|
NCSGSA |
|
3:26.78 |
- |
| 19 |
Fogleman, Ethan G (10)
|
NCSGSA |
|
4:21.99 |
- |