| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Pruden, Mary C (16)
|
NCWAVE |
53.52 |
55.99 |
2.47 |
| 2 |
Fulcher, Courtney C (17)
|
NCWAVE |
54.43 |
58.21 |
3.78 |
| 3 |
Zhang, Kara A (15)
|
NCWAVE |
56.22 |
58.96 |
2.74 |
| 4 |
Fullerton, Mikaela C (15)
|
NCWAVE |
56.61 |
59.42 |
2.81 |
| 5 |
Edelson, Anna E (15)
|
NCWAVE |
58.03 |
59.54 |
1.51 |
| 6 |
Hurlbert, Katherine R (15)
|
NCWAVE |
58.71 |
1:00.40 |
1.69 |
| 7 |
Baldwin, LeighAnne N (15)
|
NCWAVE |
57.48 |
1:01.60 |
4.12 |
| 8 |
Churchill, Andi L (18)
|
SCMBR |
57.56 |
1:01.98 |
4.42 |
| 9 |
Galloway, Claire E (15)
|
NCWAVE |
1:02.36 |
1:02.94 |
0.58 |
|
Flaherty, Danielle M (16)
|
SCMBR |
56.85 |
NS |
- |