| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Fortner, Abigail K (12)
|
NCWAVE |
31.25 |
30.33 |
-0.92 |
| 2 |
Balbuena, Gabriela C (12)
|
NCWAVE |
32.03 |
31.64 |
-0.39 |
| 3 |
Hall, Zion D (11)
|
NCWAVE |
35.66 |
35.38 |
-0.28 |
| 4 |
Paleocrassas, Katerina E (12)
|
NCWAVE |
|
36.13 |
- |
| 5 |
Habib, Ella R (11)
|
SCMBR |
37.30 |
37.21 |
-0.09 |
| 6 |
Speir, Ella N (11)
|
SCMBR |
38.79 |
38.00 |
-0.79 |
| 7 |
Holt, Hailey C (11)
|
NCWAVE |
39.37 |
39.35 |
-0.02 |
| 8 |
Rouser, Kennedy R (12)
|
NCWAVE |
39.40 |
42.06 |
2.66 |
| 9 |
Sarmiere, Alexa P (11)
|
NCWAVE |
49.10 |
43.73 |
-5.37 |
| 10 |
Ouellette, Mia G (11)
|
NCGFSC |
52.80 |
54.16 |
1.36 |
| 11 |
Brown, Miller G (11)
|
NCWAVE |
45.30 |
54.35 |
9.05 |
|
Aghoram, Aditi (11)
|
NCWAVE |
|
DQ |
- |
|
McWhorter, Kylie A (11)
|
SCMBR |
45.38 |
DQ |
- |