| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Haney, Kenna L (15)
|
NCWAVE |
24.51 |
24.65 |
0.14 |
| 2 |
Pruden, Mary C (16)
|
NCWAVE |
24.77 |
25.32 |
0.55 |
| 3 |
Fulcher, Courtney C (17)
|
NCWAVE |
24.49 |
26.02 |
1.53 |
| 4 |
Lipscomb, Madison R (16)
|
NCWAVE |
25.69 |
26.78 |
1.09 |
| 5 |
Zhang, Kara A (15)
|
NCWAVE |
26.42 |
27.16 |
0.74 |
| 6 |
Edelson, Anna E (15)
|
NCWAVE |
26.68 |
27.20 |
0.52 |
| 7 |
Fullerton, Mikaela C (15)
|
NCWAVE |
26.28 |
27.36 |
1.08 |
| 8 |
Baldwin, LeighAnne N (15)
|
NCWAVE |
26.41 |
27.54 |
1.13 |
| 9 |
Hurlbert, Katherine R (15)
|
NCWAVE |
26.64 |
27.65 |
1.01 |
| 10 |
Galloway, Claire E (15)
|
NCWAVE |
27.95 |
27.93 |
-0.02 |
| 11 |
Flaherty, Danielle M (16)
|
SCMBR |
26.78 |
28.67 |
1.89 |
| 12 |
Churchill, Andi L (18)
|
SCMBR |
28.74 |
29.10 |
0.36 |
| 13 |
Smith, Ellie A (16)
|
NCWAVE |
29.94 |
29.39 |
-0.55 |
| 14 |
Williams, Jade E (16)
|
NCWAVE |
33.75 |
34.36 |
0.61 |