| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hughes, Jackson C (10)
|
NCWOW |
42.87 |
42.17 |
-0.70 |
| 2 |
Zapler, Ezra N (9)
|
NCECA |
45.83 |
44.95 |
-0.88 |
| 3 |
Roth, Matej E (10)
|
NCECA |
46.01 |
46.35 |
0.34 |
| 4 |
Marlow, Tiago V (10)
|
NCECA |
50.14 |
49.21 |
-0.93 |
| 5 |
Kinlan, Logan T (10)
|
NCECA |
51.22 |
50.01 |
-1.21 |
| 6 |
Petroff, Behrett B (7)
|
NCWOW |
54.52 |
51.46 |
-3.06 |
| 7 |
Tillery, Wade H (10)
|
NCWOW |
52.71 |
51.67 |
-1.04 |
| 8 |
Wang, Maximilian (10)
|
NCWOW |
52.13 |
51.96 |
-0.17 |
| 9 |
Hawthorne, Maxwell S (9)
|
NCWOW |
55.79 |
52.60 |
-3.19 |
| 10 |
Witmer, William C (10)
|
NCWOW |
1:01.20 |
54.58 |
-6.62 |
| 11 |
Bollenbacher, Stephen W (9)
|
NCWOW |
|
54.94 |
- |
| 12 |
Mejouev, Maxim (10)
|
NCWOW |
54.76 |
55.68 |
0.92 |
| 13 |
Reiniche, Ryan J (9)
|
NCWOW |
1:02.29 |
56.08 |
-6.21 |
| 14 |
Roberts, Hudson P (10)
|
NCWOW |
1:00.80 |
59.16 |
-1.64 |
| 15 |
Mitchell, Everett E (9)
|
NCECA |
|
59.91 |
- |
| 16 |
Williamson, Wesley C (10)
|
NCECA |
|
1:07.50 |
- |
|
Pace, Andrew N (10)
|
NCBAC |
1:17.43 |
DQ |
- |
|
Brown, Noah D (10)
|
NCWOW |
46.77 |
NS |
- |
|
Hayes, Madden D (10)
|
NCBAC |
|
NS |
- |
|
Metty, Anderson T (10)
|
NCBAC |
46.77 |
NS |
- |