| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cassady, Hunter W (15)
|
VAYMST |
50.29 |
50.84 |
0.55 |
| 1 |
Givens-Keatley, Liam H (16)
|
NCCAST |
54.42 |
54.78 |
0.36 |
| 1 |
Ploplis, Reilly M (16)
|
NCRACY |
|
57.21 |
- |
| 2 |
Gouge, Matthew S (15)
|
NCRACY |
1:06.15 |
1:05.55 |
-0.60 |
| 2 |
Ostrander, Benjamin R (16)
|
NCRACY |
51.36 |
51.61 |
0.25 |
| 2 |
Warner, Gabriel K (16)
|
NCLOY |
54.59 |
54.82 |
0.23 |
| 3 |
Abreu, Derik (18)
|
NCCAST |
1:12.52 |
1:14.50 |
1.98 |
| 3 |
Walker, Gabe J (15)
|
NCNUMA |
53.37 |
54.48 |
1.11 |
| 3 |
Warner, Calvin I (16)
|
NCLOY |
56.27 |
55.48 |
-0.79 |
| 4 |
Carsten, Jace D (15)
|
NCLOY |
56.20 |
55.68 |
-0.52 |
| 5 |
Jernigan, Tanner A (15)
|
NCRMY |
57.17 |
55.87 |
-1.30 |
|
Dickinson, Austin T (16)
|
NCLOY |
55.35 |
NS |
- |
|
McAllister, Ryan D (17)
|
NCLOY |
1:03.02 |
NS |
- |