| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Thompson, William E (11)
|
NCACCH |
29.99 |
30.12 |
0.13 |
| 1 |
Yeh, Alexander T (12)
|
NCLOY |
35.29 |
33.28 |
-2.01 |
| 1 |
Kafitz, Christopher S (11)
|
NCRACY |
36.78 |
35.48 |
-1.30 |
| 1 |
Gayford, Zachary M (12)
|
NCLOY |
41.10 |
38.16 |
-2.94 |
| 2 |
Schott, Ayden C (12)
|
NCLOY |
38.04 |
36.43 |
-1.61 |
| 2 |
Proctor, Hunter G (11)
|
NCRMY |
39.46 |
43.35 |
3.89 |
| 3 |
Winham, Gabriel L (11)
|
NCRMY |
37.37 |
37.35 |
-0.02 |
| 3 |
Buechler, Ty W (12)
|
NCRACY |
44.35 |
43.63 |
-0.72 |
| 4 |
Little, Lucas E (11)
|
NCHAC |
48.45 |
43.69 |
-4.76 |
| 5 |
Brown, Kadin S (11)
|
NCHAC |
50.02 |
44.38 |
-5.64 |
| 6 |
Nicolet, Foster J (11)
|
NCNUMA |
45.74 |
44.78 |
-0.96 |
| 7 |
Sioussat, Stedman (12)
|
NCYBAC |
43.86 |
46.73 |
2.87 |
| 8 |
Catalano, Rocco I (12)
|
NCRMY |
48.40 |
46.88 |
-1.52 |
| 9 |
Williams, Hatcher M (11)
|
NCACCH |
46.71 |
46.96 |
0.25 |
| 10 |
Herlocker, Jaxon S (11)
|
NCRACY |
|
47.61 |
- |
| 11 |
Walsh, William C (11)
|
NCACCH |
49.97 |
49.03 |
-0.94 |
| 12 |
Pattison, Jason M (11)
|
NCHAC |
46.18 |
50.81 |
4.63 |
| 13 |
Brouwer, Ethan G (12)
|
NCNUMA |
|
51.28 |
- |
|
Kashmer, Christian J (11)
|
NCCAST |
55.64 |
DQ |
- |
|
Fike, Alexander C (12)
|
NCRACY |
|
DQ |
- |