| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Campbell, Sydney K (6)
|
PVMSSC |
49.33 |
40.36 |
-8.97 |
| 2 |
Bishop, Alexandra (Lexie) M (6)
|
PVASA |
42.36 |
41.99 |
-0.37 |
| 3 |
Tsarni, Kesi R (6)
|
PVRMSC |
|
47.00 |
- |
| 4 |
Jarczynski, Ashley N (6)
|
PVRMSC |
|
49.88 |
- |
| 5 |
Chen, Zoie (6)
|
PVRMSC |
1:06.29 |
52.57 |
-13.72 |
| 6 |
Zhou, Elly B (6)
|
PVRMSC |
|
53.66 |
- |
| 7 |
Bartholomew, Harper L (5)
|
PVRMSC |
|
55.26 |
- |
| 8 |
Bouamri, Sarah M (6)
|
PVRMSC |
|
58.04 |
- |
| 9 |
Drummond, Sophia G (6)
|
PVMSSC |
|
1:00.18 |
- |
| 10 |
Kunkle, Caroline J (6)
|
PVRMSC |
|
1:03.04 |
- |
| 11 |
Egwim, Tessa N (6)
|
PVRMSC |
|
1:10.90 |
- |
| 12 |
Groves, Charlotte (4)
|
PVASA |
1:24.81 |
1:22.27 |
-2.54 |