| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bishop, Alexandra (Lexie) M (6)
|
PVASA |
24.52 |
23.73 |
-0.79 |
| 2 |
Tsarni, Kesi R (6)
|
PVRMSC |
28.38 |
26.64 |
-1.74 |
| 3 |
Bartholomew, Harper L (5)
|
PVRMSC |
28.27 |
27.16 |
-1.11 |
| 4 |
Wofford, Rayna R (6)
|
PVRMSC |
27.83 |
27.83 |
0.00 |
| 5 |
Bouamri, Sarah M (6)
|
PVRMSC |
30.62 |
28.07 |
-2.55 |
| 6 |
Chen, Zoie (6)
|
PVRMSC |
27.44 |
29.05 |
1.61 |
| 7 |
Martin, Amelia B (6)
|
PVASA |
36.78 |
30.93 |
-5.85 |
| 8 |
Jarczynski, Ashley N (6)
|
PVRMSC |
29.52 |
31.58 |
2.06 |
| 9 |
Brammer, Ava A (6)
|
PVRMSC |
32.13 |
31.64 |
-0.49 |
| 10 |
Kunkle, Caroline J (6)
|
PVRMSC |
27.87 |
31.84 |
3.97 |
| 11 |
Harvey, Ellis A (6)
|
PVRMSC |
32.72 |
31.89 |
-0.83 |
| 12 |
Farias, Maria B (6)
|
PVMSSC |
|
33.71 |
- |
| 13 |
Wofford, Elise J (5)
|
PVRMSC |
33.69 |
33.77 |
0.08 |
| 14 |
Egwim, Tessa N (6)
|
PVRMSC |
34.72 |
34.45 |
-0.27 |
| 15 |
Drummond, Sophia G (6)
|
PVMSSC |
|
36.75 |
- |
| 16 |
Zhou, Elly B (6)
|
PVRMSC |
32.67 |
36.83 |
4.16 |
| 17 |
Groves, Charlotte (4)
|
PVASA |
41.93 |
45.52 |
3.59 |
| 18 |
Hernandez Fuentes, Ashley C (6)
|
PVRMSC |
|
48.09 |
- |
|
Campbell, Sydney K (6)
|
PVMSSC |
24.51 |
DQ |
- |