| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bacon, Phoebe M (14)
|
PVNCAP |
53.88 |
53.94 |
0.06 |
| 2 |
Gellineau, Briana N (14)
|
MRLIAC |
58.45 |
59.39 |
0.94 |
| 3 |
Higgins, Makenzie S (14)
|
PVNCAP |
58.47 |
59.93 |
1.46 |
| 4 |
Liu, Amanda (14)
|
PVRMSC |
1:00.66 |
1:00.68 |
0.02 |
| 5 |
Johnson, Jillian V (14)
|
PVNCAP |
58.88 |
1:00.97 |
2.09 |
| 6 |
Paoletti, Isabella N (14)
|
MADST |
1:00.38 |
1:01.46 |
1.08 |
| 7 |
Cosgrove, Kathleen G (14)
|
PVRMSC |
59.92 |
1:01.71 |
1.79 |
| 8 |
Borjigin, Moshelle (14)
|
PVRMSC |
59.90 |
1:01.82 |
1.92 |
| 9 |
Russell, Abigail G (14)
|
VANOVA |
1:01.80 |
1:02.93 |
1.13 |
| 10 |
Isley, Courtney A (14)
|
VANOVA |
59.23 |
1:03.25 |
4.02 |
| 11 |
Hong, Katie (14)
|
MRLIAC |
1:04.85 |
1:04.55 |
-0.30 |
| 12 |
Thomas, Avery L (14)
|
MADST |
1:07.58 |
1:07.03 |
-0.55 |
| 13 |
Nowak, Nicole (14)
|
MRLIAC |
1:04.64 |
1:07.11 |
2.47 |
|
Burton, Morgan T (14)
|
VAUN |
59.22 |
1:00.96 |
1.74 |
|
Defonte, Isabella (14)
|
MRUN |
1:03.18 |
1:02.72 |
-0.46 |