| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cottle, Elizabeth A (15)
|
FLSTAR |
56.78 |
56.22 |
-0.56 |
| 2 |
Broshears, Elizabeth G (14)
|
INBOON |
56.41 |
56.62 |
0.21 |
| 3 |
Aurnou-Rhees, Jordan W (14)
|
OHOSSC |
56.92 |
56.83 |
-0.09 |
| 4 |
Ritz, Erin (15)
|
NIVICT |
58.59 |
57.75 |
-0.84 |
| 5 |
Gallamore, Natalie G (17)
|
MIFLY |
56.35 |
58.23 |
1.88 |
| 6 |
Gaushell, Mary H (16)
|
SEMTAC |
2.15 |
58.31 |
56.16 |
| 7 |
Willis, Sydney A (16)
|
NCWAVE |
59.25 |
58.32 |
-0.93 |
| 8 |
Passilla, Sarah L (17)
|
FLSTAR |
58.79 |
59.58 |
0.79 |
| 9 |
Rodriguez, Jessica (17)
|
FGSOFL |
1:01.68 |
59.89 |
-1.79 |
| 10 |
Bowers, Anne C (18)
|
NCASC |
59.20 |
1:00.17 |
0.97 |
| 11 |
Tolan, Ann E (17)
|
MELRSC |
57.00 |
1:00.27 |
3.27 |
| 12 |
Gordon, Lindsey S (17)
|
CTRAC |
1:01.49 |
1:00.49 |
-1.00 |
| 13 |
Klafehn, Bailey R (17)
|
NIVICT |
58.99 |
1:00.56 |
1.57 |
| 14 |
Rodriguez, Mayrangela (16)
|
FLSTAR |
59.68 |
1:00.60 |
0.92 |
| 15 |
Payne, Grayson E (14)
|
SEMCC |
58.99 |
1:00.79 |
1.80 |
| 16 |
Divoky, Brenna E (15)
|
NCWOW |
1:01.81 |
1:01.59 |
-0.22 |
| 17 |
Windheim, Jenna M (16)
|
NIFAST |
1:04.75 |
1:04.86 |
0.11 |