| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ritz, Jared P (17)
|
NIVICT |
22.17 |
23.00 |
0.83 |
| 2 |
Smith, Tyler N (16)
|
NIUN-V |
22.86 |
23.76 |
0.90 |
| 3 |
Regelsberger, Tyler R (16)
|
NIUN-V |
22.43 |
23.97 |
1.54 |
| 4 |
Thomas, Joshua D (15)
|
NIOLSC |
24.71 |
24.63 |
-0.08 |
| 5 |
McEwen, Andrew S (17)
|
NIVICT |
22.92 |
24.73 |
1.81 |
| 6 |
Bethlendy, Liam G (17)
|
NIRRST |
23.80 |
24.83 |
1.03 |
| 7 |
Bischoping, Tyler J (16)
|
NIGCOM |
25.73 |
25.30 |
-0.43 |
| 8 |
Bushen, Isaac J (15)
|
NIGCOM |
26.30 |
25.33 |
-0.97 |
| 9 |
Martello, Mitchell J (17)
|
NIVICT |
25.82 |
25.84 |
0.02 |
| 10 |
Teator, Jonathan J (17)
|
NISDSC |
25.43 |
26.45 |
1.02 |
| 11 |
Stone, Parker D (17)
|
NIGCOM |
24.86 |
26.80 |
1.94 |