| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Haney, Kenna L (15)
|
NCWAVE |
34.10 |
33.41 |
-0.69 |
| 2 |
Hoover, Madison M (15)
|
NCWOW |
36.81 |
34.81 |
-2.00 |
| 3 |
Evans, Alyssa B (15)
|
NCWAVE |
37.43 |
37.09 |
-0.34 |
| 4 |
Edelson, Anna E (15)
|
NCWAVE |
37.24 |
37.60 |
0.36 |
| 5 |
Fullerton, Mikaela C (16)
|
NCWAVE |
40.09 |
38.15 |
-1.94 |
| 5 |
Johnson, Emma (15)
|
NCSSS |
40.32 |
38.15 |
-2.17 |
| 7 |
Chando, Terese E (16)
|
NCUNWA |
39.60 |
38.47 |
-1.13 |
| 8 |
Hurlbert, Katherine R (15)
|
NCWAVE |
37.57 |
39.00 |
1.43 |
| 9 |
Hyldahl, Aubrey E (15)
|
NCWOW |
41.29 |
39.34 |
-1.95 |
| 10 |
Galloway, Claire E (15)
|
NCWAVE |
41.02 |
40.44 |
-0.58 |
| 11 |
Jewell, Aleta M (18)
|
NCWOW |
37.82 |
42.04 |
4.22 |
| 12 |
Ball, Maureen E (15)
|
NCMOR |
51.97 |
43.93 |
-8.04 |
|
Jewell, Brynn H (16)
|
NCWOW |
37.00 |
DQ |
- |
|
Greene, Dana M (48)
|
NCWAVE |
|
NS |
- |