| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Thorpe, Braelyn O (6)
|
FGCSSC |
24.65 |
23.10 |
-1.55 |
| 2 |
Mirones, Lilliana M (6)
|
FGSUN |
28.25 |
26.92 |
-1.33 |
| 3 |
Jorgensen, Kierstyn E (6)
|
FGSUN |
30.75 |
27.75 |
-3.00 |
| 4 |
Evans, Shelby O (6)
|
FGCCPR |
26.47 |
29.06 |
2.59 |
| 5 |
Evans, Jordan C (5)
|
FGCCPR |
27.24 |
29.35 |
2.11 |
| 6 |
DiSarno, Naiyla F (6)
|
FGSUN |
|
30.61 |
- |
| 7 |
Rodriguez Walker, Vanessa L (6)
|
FGCSSC |
|
30.70 |
- |
| 8 |
Harding, Aveline (6)
|
FGCSSC |
37.82 |
32.41 |
-5.41 |
| 9 |
Hillebert, Emma R (6)
|
FGCCPR |
45.67 |
32.71 |
-12.96 |
| 10 |
Jean-Baptiste, Daneika (5)
|
FGSUN |
|
41.53 |
- |
| 11 |
Landron, Veronica L (4)
|
FGSUN |
53.06 |
1:04.26 |
11.20 |
|
Martin, Gianna S (6)
|
FGCSSC |
|
NS |
- |
|
Samansky, Jordan L (5)
|
FGHAFL |
|
NS |
- |
|
Rilo, Athena (6)
|
FGSUN |
35.20 |
NS |
- |