| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Thorpe, Braelyn O (6)
|
FGCSSC |
23.03 |
21.22 |
-1.81 |
| 2 |
LaPietra, Sabina S (6)
|
FGPST |
23.50 |
22.48 |
-1.02 |
| 3 |
Mirones, Lilliana M (6)
|
FGSUN |
25.81 |
26.96 |
1.15 |
| 4 |
Evans, Shelby O (6)
|
FGCCPR |
27.52 |
27.58 |
0.06 |
| 5 |
Martin, Gianna S (6)
|
FGCSSC |
32.28 |
27.91 |
-4.37 |
| 6 |
Harding, Aveline (6)
|
FGCSSC |
31.93 |
28.98 |
-2.95 |
| 7 |
Rodriguez Walker, Vanessa L (6)
|
FGCSSC |
34.39 |
29.08 |
-5.31 |
| 8 |
Evans, Jordan C (6)
|
FGCCPR |
29.10 |
29.63 |
0.53 |
| 9 |
LaPietra, Wendy G (4)
|
FGPST |
|
30.61 |
- |
| 10 |
Santos, Sophia T (6)
|
FGPCS |
|
32.15 |
- |
| 11 |
Rilo, Athena (6)
|
FGSUN |
32.40 |
33.96 |
1.56 |
| 12 |
Jorgensen, Kierstyn E (6)
|
FGSUN |
33.55 |
33.97 |
0.42 |
| 13 |
Larsen, Sydney R (6)
|
FGCSSC |
44.75 |
42.30 |
-2.45 |
| 14 |
Jean-Baptiste, Daneika (5)
|
FGSUN |
52.05 |
45.93 |
-6.12 |
| 15 |
Swirbel, Ashley G (5)
|
FGHAFL |
|
1:05.30 |
- |