| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wagner, Morgan E (10)
|
NIVICT |
33.37 |
30.58 |
-2.79 |
| 2 |
Prezyna, Zoey (10)
|
NIVICT |
31.49 |
31.50 |
0.01 |
| 3 |
Natemeier, Amelia E (10)
|
NIUN-V |
31.61 |
31.68 |
0.07 |
| 4 |
Gachette, Marisa B (10)
|
NIVELO |
32.74 |
33.31 |
0.57 |
| 5 |
Anderson, Madden (10)
|
NIVELO |
34.10 |
33.48 |
-0.62 |
| 6 |
Barillari, Emma (10)
|
NIVELO |
34.82 |
34.39 |
-0.43 |
| 7 |
King, Skylar L (10)
|
NIEAST |
35.45 |
34.75 |
-0.70 |
| 8 |
Philbrick, Mylie E (10)
|
NIVELO |
34.49 |
35.06 |
0.57 |
| 9 |
Cywinski, Anna C (9)
|
NIVICT |
34.93 |
35.32 |
0.39 |
| 10 |
Reilly, Bridget F (9)
|
NISTAR |
34.70 |
36.16 |
1.46 |
| 11 |
Reukauf, Anna R (10)
|
NISTAR |
37.05 |
36.92 |
-0.13 |
| 12 |
Attuwaybi, Eman (9)
|
NIVELO |
42.93 |
41.77 |
-1.16 |
| 13 |
Rasmusson, Henrietta E (9)
|
NIUN-C |
41.11 |
42.04 |
0.93 |
| 14 |
Arcadi, Kayla A (10)
|
NIVELO |
|
43.01 |
- |
| 15 |
Burnham, Madeline (9)
|
NIVELO |
44.83 |
43.96 |
-0.87 |
| 16 |
Crozier, Sydney (10)
|
NIVELO |
42.32 |
44.36 |
2.04 |
| 17 |
Wands, Lucille M (9)
|
NIVELO |
40.25 |
44.46 |
4.21 |
| 18 |
Washington, Ashanti M (9)
|
NISTAR |
51.07 |
45.57 |
-5.50 |
| 19 |
Parzymieso, Megan A (9)
|
NISTAR |
|
1:01.75 |
- |