| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wisner, Taryn A (14)
|
NIVICT |
53.04 |
56.22 |
3.18 |
| 2 |
Blake, Emily C (13)
|
NISTAR |
58.23 |
57.79 |
-0.44 |
| 3 |
Heltz, Olivia G (14)
|
NISTAR |
58.25 |
1:00.01 |
1.76 |
| 4 |
Bray, Karly T (14)
|
NISTAR |
59.17 |
1:00.19 |
1.02 |
| 5 |
McMullen, Haley (13)
|
NIVELO |
1:08.48 |
1:01.25 |
-7.23 |
| 6 |
Pathammavong, Suriyathai A (13)
|
NIVICT |
1:00.89 |
1:01.90 |
1.01 |
| 7 |
Yang, Ellia (14)
|
NIVELO |
1:00.89 |
1:02.02 |
1.13 |
| 8 |
Hall, Makaila (13)
|
NIVELO |
1:06.03 |
1:02.57 |
-3.46 |
| 9 |
Kanner, Sydney G (14)
|
NISTAR |
1:06.70 |
1:03.74 |
-2.96 |
| 10 |
Rhodes, Maggie (13)
|
CCSC |
1:07.32 |
1:04.64 |
-2.68 |
| 11 |
DeMeo, Isabella B (14)
|
NISTAR |
1:07.85 |
1:05.12 |
-2.73 |
| 12 |
Rietz, Tarin (13)
|
NIVELO |
1:08.27 |
1:05.44 |
-2.83 |
| 13 |
Sousis, Gabriella R (14)
|
NISTAR |
1:08.99 |
1:05.99 |
-3.00 |
| 14 |
Gentile, Juliana (14)
|
NIVELO |
1:08.06 |
1:06.93 |
-1.13 |
| 15 |
Xu, Emily (14)
|
NIVELO |
|
1:10.37 |
- |
| 16 |
Randall, Alexandra E (14)
|
NISHAQ |
1:13.35 |
1:11.25 |
-2.10 |
| 17 |
Utz, Natalie R (13)
|
NIGCOM |
1:17.33 |
1:13.09 |
-4.24 |
| 18 |
Langlotz, Olivia M (13)
|
NICLSC |
1:36.09 |
1:19.06 |
-17.03 |
| 19 |
Asher, Sophia (13)
|
NIVELO |
1:25.88 |
1:19.90 |
-5.98 |
| 20 |
Delille, Lorreine (14)
|
NIVELO |
|
1:21.49 |
- |
| 21 |
Canestaro, Annabelle S (13)
|
NIVELO |
1:29.20 |
1:25.84 |
-3.36 |
|
Rath, Cecilia R (14)
|
NICLSC |
|
NS |
- |