| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Keller, Mira (11)
|
NIVELO |
31.77 |
30.68 |
-1.09 |
| 2 |
Griffin, Zoey O (11)
|
NICLSC |
33.35 |
32.75 |
-0.60 |
| 3 |
Amer, Aya F (12)
|
NIVELO |
34.34 |
34.70 |
0.36 |
| 4 |
Skenderija, Sofija (12)
|
NIUN-D |
45.00 |
35.36 |
-9.64 |
| 5 |
Betzig, Brooklynn K (11)
|
NISTAR |
|
37.95 |
- |
| 6 |
Lin, Isabella E (12)
|
NIVELO |
36.68 |
38.12 |
1.44 |
| 7 |
Wands, Amelia G (11)
|
NIVELO |
38.46 |
38.71 |
0.25 |
| 8 |
Gentile, Olivia (11)
|
NIVELO |
43.29 |
41.76 |
-1.53 |
| 9 |
Tripi, Haylee E (12)
|
NIVELO |
43.30 |
42.03 |
-1.27 |
| 10 |
Eisenmann, Abigail E (12)
|
NISTAR |
44.13 |
42.74 |
-1.39 |
| 11 |
Moldenhauer, Paige A (11)
|
NIEAST |
46.66 |
43.11 |
-3.55 |
| 12 |
Seelig, Jenna N (11)
|
NICLSC |
47.94 |
43.72 |
-4.22 |
| 13 |
Chelpinski, Angelica J (12)
|
NIVELO |
46.68 |
44.06 |
-2.62 |
| 14 |
Battistoni, Grace L (11)
|
NIVELO |
44.23 |
44.17 |
-0.06 |
| 15 |
Delille, Beatrice F (12)
|
NIVELO |
|
44.52 |
- |
| 16 |
Booth, Sophia A (11)
|
NISTAR |
50.92 |
45.40 |
-5.52 |
| 17 |
Harrison, Ella C (11)
|
NICLSC |
54.15 |
46.03 |
-8.12 |
| 18 |
Monteiro, Martha G (11)
|
NIVELO |
52.63 |
46.56 |
-6.07 |
| 19 |
DelRegno, Isabella M (11)
|
NIVELO |
51.27 |
49.43 |
-1.84 |
| 20 |
Meade, Juliana E (11)
|
NISTAR |
57.21 |
49.71 |
-7.50 |
| 21 |
Thurston, Lauren A (11)
|
NIGCOM |
53.66 |
57.83 |
4.17 |