| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wagner, Morgan E (10)
|
NIVICT |
2:27.27 |
2:23.64 |
-3.63 |
| 2 |
Cywinski, Anna C (9)
|
NIVICT |
2:42.47 |
2:42.10 |
-0.37 |
| 3 |
Gachette, Marisa B (10)
|
NIVELO |
2:57.63 |
2:43.56 |
-14.07 |
| 4 |
Philbrick, Mylie E (10)
|
NIVELO |
3:07.40 |
2:44.79 |
-22.61 |
| 5 |
Strittmatter, Amelia C (10)
|
NISTAR |
3:06.97 |
2:46.20 |
-20.77 |
| 6 |
Crossing, Natalie E (10)
|
NIVICT |
2:50.82 |
2:49.41 |
-1.41 |
| 7 |
Barillari, Emma (10)
|
NIVELO |
3:03.71 |
2:54.34 |
-9.37 |
| 8 |
Reukauf, Anna R (10)
|
NISTAR |
3:15.88 |
3:11.85 |
-4.03 |
| 9 |
Tothero, Madeleine S (10)
|
NISTAR |
3:51.12 |
3:25.26 |
-25.86 |
| 10 |
King, Izzy M (9)
|
NIEAST |
3:52.41 |
3:33.22 |
-19.19 |