| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Haley, Jack (10)
|
CTCT |
26.41 |
26.65 |
0.24 |
| 2 |
Eckerstrom, Gunnar R (10)
|
NJNJS |
26.67 |
27.14 |
0.47 |
| 3 |
Stuek, Lincoln B (10)
|
NJNJS |
27.45 |
27.22 |
-0.23 |
| 4 |
Kulp, Charlie J (10)
|
NJNJS |
27.05 |
27.35 |
0.30 |
| 5 |
Bradshaw, Ryan B (10)
|
PVPV |
27.39 |
27.51 |
0.12 |
| 6 |
Kabunov, Mikhail V (10)
|
NINI |
28.37 |
27.54 |
-0.83 |
| 7 |
Cakir, Noah (9)
|
MRMETR |
27.95 |
27.67 |
-0.28 |
| 8 |
Czartoryjski, Damian (10)
|
CTCT |
27.28 |
27.73 |
0.45 |
| 9 |
Hwang, Ryan (10)
|
MRMETR |
28.26 |
27.89 |
-0.37 |
| 10 |
Tain, Connor P (10)
|
PVPV |
28.00 |
27.99 |
-0.01 |
| 11 |
Piccin, Alexander (10)
|
CTCT |
28.35 |
28.50 |
0.15 |
| 12 |
Char, Andrew T (10)
|
PVPV |
28.84 |
28.63 |
-0.21 |
| 13 |
Mendoza, Aaron O (10)
|
MRMETR |
28.51 |
29.04 |
0.53 |
| 14 |
Durr, Tucker C (10)
|
ADAD |
29.23 |
29.18 |
-0.05 |
| 15 |
Cooper, Soren K (10)
|
AMAM |
29.34 |
29.24 |
-0.10 |
| 16 |
Tucker, Justin K (10)
|
AMAM |
29.28 |
29.52 |
0.24 |
| 17 |
Powell, Trenten J (10)
|
AMAM |
30.40 |
29.90 |
-0.50 |
| 18 |
Borden, Jonas O (10)
|
NINI |
29.92 |
30.21 |
0.29 |
| 19 |
Fan, Timothy (10)
|
NINI |
30.47 |
30.39 |
-0.08 |
| 20 |
Staff, Brody J (10)
|
ADAD |
30.59 |
30.50 |
-0.09 |