| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Murphy, Abbie (11)
|
NIVICT |
36.63 |
36.54 |
-0.09 |
| 2 |
Natemeier, Amelia E (11)
|
NIVICT |
39.14 |
39.56 |
0.42 |
| 3 |
Sangasy, KhaNy K (12)
|
NIGCOM |
40.65 |
39.61 |
-1.04 |
| 4 |
Adamczyk, Gabrielle J (11)
|
NICLSC |
40.58 |
41.20 |
0.62 |
| 5 |
Kleinhans, Reese E (12)
|
NIMERC |
42.64 |
41.62 |
-1.02 |
| 6 |
Caldwell, Hannah M (11)
|
NIVICT |
41.84 |
42.78 |
0.94 |
| 7 |
Hersh, Erin (11)
|
NIVICT |
46.09 |
47.14 |
1.05 |
| 8 |
Kazmark, Olivia N (12)
|
NIVICT |
47.18 |
47.20 |
0.02 |
| 9 |
Rozalski, Jasmine L (12)
|
NIMERC |
49.24 |
47.61 |
-1.63 |
| 10 |
Kenny, Elisabeth A (12)
|
NIQUIK |
50.23 |
50.04 |
-0.19 |
| 11 |
Thurston, Lauren A (11)
|
NIGCOM |
54.71 |
51.60 |
-3.11 |
| 12 |
Campbell, Brooklyn M (12)
|
NIVICT |
58.87 |
52.59 |
-6.28 |
| 13 |
Stefanou, Ava C (11)
|
NIQUIK |
1:00.85 |
58.12 |
-2.73 |
| 14 |
Zeller, Sara L (12)
|
NISDSC |
1:06.15 |
1:00.36 |
-5.79 |
| 15 |
Gucker, Margaret A (11)
|
NIRRST |
|
1:00.72 |
- |
|
Seelig, Jenna N (11)
|
NICLSC |
45.46 |
NS |
- |