| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Tarafa, Matthew A (12)
|
FGHSC |
23.27 |
23.38 |
0.11 |
| 2 |
Rodriguez, Agustin F (12)
|
FGHSC |
24.98 |
24.72 |
-0.26 |
| 3 |
Hewitt, Carter M (12)
|
FGECAC |
26.28 |
25.25 |
-1.03 |
| 3 |
Bishop, Hudson T (12)
|
FGECAC |
26.41 |
25.25 |
-1.16 |
| 5 |
Justin, Joshua J (12)
|
FGPST |
26.32 |
25.81 |
-0.51 |
| 6 |
Chavez, David J (12)
|
FGMS |
26.64 |
25.94 |
-0.70 |
| 7 |
Rueda, Ronny B (12)
|
FGMLB |
26.41 |
26.03 |
-0.38 |
| 8 |
Pujula, Charles (12)
|
FGHSC |
26.65 |
26.05 |
-0.60 |
| 9 |
Yost, David S (12)
|
FGECAC |
26.20 |
26.31 |
0.11 |
| 10 |
Rodriguez, Enrique (12)
|
FGSOFL |
26.52 |
26.34 |
-0.18 |
| 11 |
Smiley, Dylan (12)
|
FGMCDA |
26.54 |
26.36 |
-0.18 |
| 12 |
Curtis, Steven M (11)
|
FGJDST |
26.84 |
26.42 |
-0.42 |
| 13 |
Trieu, John T (11)
|
FGFAST |
26.45 |
26.42 |
-0.03 |
| 14 |
Farrell, Liam P (12)
|
FGJDST |
27.78 |
26.75 |
-1.03 |
| 15 |
Lopez-Casula, Marcoaurelio (12)
|
FGHSC |
27.35 |
26.90 |
-0.45 |
| 16 |
Echeumuna, Luca A (11)
|
FGAZFL |
27.08 |
26.94 |
-0.14 |
| 16 |
Ajo, Henry (11)
|
FGBMHG |
27.09 |
26.94 |
-0.15 |
| 18 |
Nunez, Alex B (12)
|
FGJDST |
27.69 |
27.31 |
-0.38 |
| 19 |
Taylor, Mason B (12)
|
FGJDST |
27.58 |
27.42 |
-0.16 |
| 20 |
Foulds, Chase D (12)
|
FGECAC |
27.60 |
27.64 |
0.04 |
| 21 |
Rosenthal, Parker A (12)
|
FGGRSC |
27.59 |
27.70 |
0.11 |
| 22 |
Felt, Dylan M (12)
|
FGSFTL |
27.20 |
27.74 |
0.54 |
| 23 |
Balbi, Julian A (12)
|
FGSAS |
27.77 |
27.78 |
0.01 |
| 24 |
Leon, Leonardo (12)
|
FGCM |
27.45 |
27.83 |
0.38 |
| 25 |
Grant, Daniel P (12)
|
FGGRSC |
27.52 |
27.94 |
0.42 |
| 26 |
Nordheim, Gregory A (12)
|
FGSAS |
27.80 |
28.03 |
0.23 |
| 27 |
Gonzalez, Logan G (12)
|
FGSOFL |
27.72 |
28.08 |
0.36 |
| 28 |
Young, Kevin T (12)
|
FGJDST |
27.80 |
28.49 |
0.69 |
|
Campos, David A (12)
|
FGGRSC |
27.38 |
DQ |
- |