| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
LaRue, Carl V (14)
|
NIQUIK |
4:58.56 |
4:41.97 |
-16.59 |
| 2 |
Evans, Rebecca L (16)
|
NIFAST |
4:29.20 |
4:45.44 |
16.24 |
| 3 |
Chen, Grace (16)
|
NIFAST |
4:54.24 |
4:54.72 |
0.48 |
| 4 |
Doescher, Mackenzi L (15)
|
NIFAST |
4:46.22 |
5:02.44 |
16.22 |
| 5 |
Wood, Alicia G (14)
|
NIIRON |
5:29.83 |
5:06.35 |
-23.48 |
| 6 |
Healy, John C (15)
|
NIWEBS |
5:41.83 |
5:12.05 |
-29.78 |
| 7 |
Nenadic, Maria G (15)
|
NIFAST |
4:52.42 |
5:18.60 |
26.18 |
| 8 |
Miller, Tyler V (13)
|
NIFAST |
5:38.60 |
5:33.72 |
-4.88 |
| 9 |
Solodova, Elena A (12)
|
NIFAST |
5:51.01 |
5:34.00 |
-17.01 |
| 10 |
VanHouten, Ellie T (13)
|
NIPACK |
5:50.44 |
5:35.16 |
-15.28 |
| 11 |
O'Shea, Connor J (15)
|
NIFAST |
6:03.44 |
5:38.91 |
-24.53 |
| 12 |
Xu, Ming Na (11)
|
NIFAST |
5:21.80 |
5:40.99 |
19.19 |
| 13 |
Golembiewski, Lucas A (10)
|
NIPACK |
|
6:03.82 |
- |
| 14 |
Fan, Timothy (10)
|
NIPACK |
|
6:22.62 |
- |
| 15 |
Magnera, Sofia A (11)
|
NIWEBS |
|
6:57.34 |
- |
|
Ryan, Cody J (15)
|
NIFAST |
5:35.48 |
DQ |
- |
|
D'Ambrosia, Ben P (11)
|
NIWEBS |
|
DQ |
- |
|
Hinz, Kaiden M (11)
|
NIWEBS |
|
NS |
- |