| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Schwandt, Natalie K (10)
|
NIGRAT |
36.69 |
35.46 |
-1.23 |
| 2 |
Knausz, Eleanor M (10)
|
NISDSC |
35.60 |
35.77 |
0.17 |
| 3 |
Maas, Kaylee A (9)
|
NIIRON |
|
36.79 |
- |
| 4 |
Munson, Sophie M (10)
|
NIGRSC |
41.17 |
37.54 |
-3.63 |
| 5 |
Buzzell, Emily H (9)
|
NIGRSC |
44.11 |
42.36 |
-1.75 |
| 6 |
Barber, Cassandra L (10)
|
NIGRSC |
|
44.48 |
- |
| 7 |
Grigg, Charlotte M (10)
|
NIGRSC |
|
45.91 |
- |
| 8 |
Marsh, Emma L (10)
|
NIGRSC |
1:00.03 |
46.47 |
-13.56 |
| 9 |
Thaney, Brigid G (9)
|
NIGRSC |
|
47.26 |
- |
| 10 |
Farina, Samantha R (10)
|
NIGRSC |
|
48.67 |
- |
| 11 |
Bub, Emily R (10)
|
NIGRSC |
54.83 |
52.24 |
-2.59 |
| 12 |
Nitti, Marissa R (10)
|
NIGRSC |
|
57.31 |
- |
| 13 |
Baum, Scarlett A (9)
|
NIGRSC |
|
1:03.19 |
- |
|
Phillips, Hayley Q (9)
|
NIIRON |
43.41 |
NS |
- |
|
Crowley, Lila M (9)
|
NIGRSC |
41.77 |
NS |
- |
|
Mack, Annabella G (10)
|
NIGCOM |
|
NS |
- |
|
Galitsky, AnnaElizabeth (10)
|
NIGCOM |
|
NS |
- |