| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Partlow, McKenzie S (11)
|
NIOLSC |
33.77 |
30.76 |
-3.01 |
| 2 |
Zerger, Belah (12)
|
NIGRSC |
33.62 |
31.93 |
-1.69 |
| 3 |
Robinson, Sara C (11)
|
NISDSC |
37.79 |
35.13 |
-2.66 |
| 4 |
Stonehouse, Sierra L (12)
|
NIGRSC |
42.25 |
37.09 |
-5.16 |
| 5 |
Buzzell, Ella L (12)
|
NIGRSC |
36.90 |
39.73 |
2.83 |
| 6 |
White, Shaelyn J (12)
|
NIUN-G |
|
42.89 |
- |
| 7 |
Hackett, Grace C (11)
|
NIIRON |
45.21 |
44.38 |
-0.83 |
| 8 |
Saysamone, Leina H (11)
|
NIGCOM |
|
44.98 |
- |
| 9 |
Raimondo, Erin E (11)
|
NIGRSC |
|
54.51 |
- |
| 10 |
Waters, Megan R (12)
|
NIGCOM |
|
58.67 |
- |
|
Thompson, Daniella J (12)
|
NIGRSC |
47.53 |
NS |
- |