| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Knausz, Eleanor M (10)
|
NISDSC |
44.09 |
42.09 |
-2.00 |
| 2 |
Resch, Rebecka J (10)
|
NIGRSC |
43.19 |
43.30 |
0.11 |
| 3 |
Hyde, Keira E (10)
|
NIIRON |
47.47 |
44.15 |
-3.32 |
| 4 |
Munson, Sophie M (10)
|
NIGRSC |
50.59 |
44.77 |
-5.82 |
| 5 |
Schwandt, Natalie K (10)
|
NIGRAT |
47.76 |
44.84 |
-2.92 |
| 6 |
Buzzell, Emily H (9)
|
NIGRSC |
51.32 |
50.08 |
-1.24 |
| 7 |
Grigg, Charlotte M (10)
|
NIGRSC |
|
50.40 |
- |
| 8 |
Bub, Emily R (10)
|
NIGRSC |
55.93 |
52.96 |
-2.97 |
| 9 |
Marsh, Emma L (10)
|
NIGRSC |
1:10.91 |
53.67 |
-17.24 |
| 10 |
Farina, Samantha R (10)
|
NIGRSC |
|
54.01 |
- |
| 11 |
Mack, Annabella G (10)
|
NIGCOM |
|
57.08 |
- |
| 12 |
Baum, Scarlett A (9)
|
NIGRSC |
|
57.36 |
- |
| 13 |
Nitti, Marissa R (10)
|
NIGRSC |
|
58.44 |
- |
| 14 |
Thaney, Brigid G (9)
|
NIGRSC |
|
1:03.22 |
- |
|
Barber, Cassandra L (10)
|
NIGRSC |
|
DQ |
- |
|
Crowley, Lila M (9)
|
NIGRSC |
51.16 |
NS |
- |
|
Phillips, Hayley Q (9)
|
NIIRON |
45.77 |
NS |
- |
|
Galitsky, AnnaElizabeth (10)
|
NIGCOM |
|
NS |
- |