| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Napier, Alexander W (10)
|
NISDSC |
50.76 |
44.58 |
-6.18 |
| 2 |
O'Dea, Daniel W (10)
|
NIGRSC |
48.65 |
45.75 |
-2.90 |
| 3 |
Kelley, Cohen R (10)
|
NIGRAT |
50.73 |
45.90 |
-4.83 |
| 4 |
Miller, Kevin T (10)
|
NIIRON |
52.72 |
46.14 |
-6.58 |
| 5 |
Soto, Giovanni A (10)
|
NIIRON |
50.12 |
46.61 |
-3.51 |
| 6 |
Richards-Dyson, Jax E (9)
|
NIGCOM |
|
47.55 |
- |
| 7 |
Panfil, Tyler J (10)
|
NIGRSC |
|
49.82 |
- |
| 8 |
Ashby, Jacob T (9)
|
NISDSC |
47.40 |
49.91 |
2.51 |
| 9 |
Ramsperger, Jacob T (9)
|
NISDSC |
51.91 |
52.52 |
0.61 |
| 10 |
Spalty, Dylan K (10)
|
NIGCOM |
|
58.54 |
- |
| 11 |
Pogroszewski, Noah Z (10)
|
NIGCOM |
|
1:00.28 |
- |
|
Cottone, Cameron A (10)
|
NIGCOM |
1:01.59 |
DQ |
- |
|
Spalty, Scott A (10)
|
NIGCOM |
|
DQ |
- |
|
Rene, Jax R (9)
|
NIIRON |
52.08 |
DQ |
- |